transfection

transfection
The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cell\'s chromosomal DNA. Usually accomplished using DNA precipitated with calcium ions though a variety of other methods can be used (eg. electroporation). Only about 1% of cultured cells are normally transfected. Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses. Though originally used to describe the situation in which the transfected DNA is integrated, it is now frequently used just to mean introduction of DNA into a target cell, hence the necessity to specify stable transfection.

Dictionary of molecular biology. 2004.

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  • Transfection — is the process of introducing nucleic acids into cells by non viral methods [http://www.promega.com/guides/transfxn guide/transfxn.pdf] . The term transformation is preferred to describe non viral DNA transfer in bacteria and non animal… …   Wikipedia

  • transfection — transfection. См. трансфекция. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • transfection — ● transfection nom féminin Introduction d un fragment d A.D.N. étranger à l intérieur d une cellule, au cours d une expérience, d un traitement par thérapie génique …   Encyclopédie Universelle

  • transfection — [trans fek′shən, tranzfek′shən] n. the injection of naked nucleic acids into cells or bacteria so as to infect them, as in cancer research transfect vt …   English World dictionary

  • Transfection — On appelle transfection le processus de transfert de gènes, c est à dire l introduction d ADN exogène dans des cellules eucaryotes, non médié par un virus, par opposition à la transduction. À noter que le terme « transfection » est… …   Wikipédia en Français

  • transfection — /trans fek sheuhn/, n. Biotech. the insertion into a cell of a bacterial plasmid that contains a foreign virus or genetic material. [1964; TRANS + (IN)FECTION] * * * ▪ biology       a term sometimes used for an infection initiated by using only… …   Universalium

  • transfection — A method of gene transfer utilizing infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell. [trans + infection] * * * trans·fec·tion …   Medical dictionary

  • transfection — noun Etymology: trans + infection Date: 1964 infection of a cell with isolated viral nucleic acid followed by production of the complete virus in the cell; also the incorporation of exogenous DNA into a cell • transfect transitive verb …   New Collegiate Dictionary

  • transfection — noun tɹænzˈfɛkʃən The introduction of foreign DNA into a eukaryotic cell. See Also: transfectant …   Wiktionary

  • transfection — trans·fec·tion …   English syllables

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